Mouse TG (Thyroglobulin) ELISA Kit | ELK1446

Mouse TG (Thyroglobulin) ELISA Kit | ELK1446 | Gentaur UK, US & Europe Distribution

Alternative Names: AITD3; TGN

Species: Mouse

Assay Type: Sandwich

Sensitivity: 13.3 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Endocrinology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroglobulin (TG) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroglobulin (TG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroglobulin (TG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroglobulin (TG) in the samples is then determined by comparing the OD of the samples to the standard curve.