749

Mouse TGF-beta receptor type-2 (TGFBR2) ELISA Kit | KTE70373

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SKU:
749-KTE70373
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€1,881.00 - €3,096.00

Description

Mouse TGF-beta receptor type-2 (TGFBR2) ELISA Kit | KTE70373 | Gentaur UK, US & Europe Distribution

Application: This Mouse TGF-beta receptor type-2 (TGFBR2) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFBR2 in samples. An antibody specific for TGFBR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGFBR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFBR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFBR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse TGF-beta receptor type-2 microplate
• Mouse TGF-beta receptor type-2 standard
• Mouse TGF-beta receptor type-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse TGF-beta receptor type-2 (TGFBR2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TGFBR2. No significant cross-reactivity or interference between Mouse TGFBR2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: TGFBR2 encodes a member of the serine/threonine protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 have been associated with Marfan syndrome, Loeys-Deitz aortic aneurysm syndrome, Osler-Weber-Rendu syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized.

Alternative Names: TGFBR2; AAT3; FAA3; LDS1B; LDS2B; MFS2; RIIC; TAAD2; TGFR-2; TGFbeta-RII; TGF-beta receptor typeB; TGF-beta type receptor; transforming growth factor beta receptor typeC; transforming growth factor; b

Search name: TGFBR2; AAT3; FAA3; LDS1B; LDS2B; MFS2; RIIC; TAAD2; TGFR-2; TGFbeta-RII; TGF-beta receptor typeB; TGF-beta type receptor; transforming growth factor beta receptor typeC; transforming growth factor; b

Tag: TGFBR2

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