Mouse TNFAIP3-interacting protein 2 (TNIP2) ELISA Kit | AE13952MO

Mouse TNFAIP3-interacting protein 2 (TNIP2) ELISA Kit | AE13952MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: TNIP2

Alternative Name: RP3-474M20.1; ABIN2; DKFZp434J1313; FLIP1; KLIP; MGC4289; A20-binding inhibitor of NF-kappaB activation 2|A20-binding inhibitor of NF-kappaB activation-2|fetal liver LKB1-interacting protein

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤5.3%

Inter-Assay: ≤7.3%

Recovery: 0, 97

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TNIP2 in samples. An antibody specific for TNIP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNIP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNIP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNIP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: TNIP2 binds to the C-terminal zinc finger domain of A20 (TNFAIP3) and is involved in activation of the ERK MAP kinase pathway in various cell types. By yeast 2-hybrid screening of a mouse fibrosarcoma cDNA library using A20 as bait, followed by database analysis, Van Huffel et al. (2001) identified human TNIP2, which they called ABIN2. The mouse and human proteins share 76% amino acid identity. Northern blot analysis of mouse tissues revealed ubiquitous expression, with highest expression in kidney and weakest expression in skeletal muscle. Western blot analysis revealed a 50-kD endogenous Abin2 protein in mouse macrophages and fibrosarcoma cells.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .