Mouse TRAF-interacting protein with FHA domain-containing protein A (TIFA) ELISA Kit | KTE71419

Mouse TRAF-interacting protein with FHA domain-containing protein A (TIFA) ELISA Kit | KTE71419 | Gentaur UK, US & Europe Distribution

Application: This Mouse TRAF-interacting protein with FHA domain-containing protein A (TIFA) ELISA Kit employs a two-site sandwich ELISA to quantitate TIFA in samples. An antibody specific for TIFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIFA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIFA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIFA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse TRAF-interacting protein with FHA domain-containing protein A microplate
• Mouse TRAF-interacting protein with FHA domain-containing protein A standard
• Mouse TRAF-interacting protein with FHA domain-containing protein A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse TRAF-interacting protein with FHA domain-containing protein A (TIFA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TIFA. No significant cross-reactivity or interference between Mouse TIFA and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: IL-22, a class of potent mediators of cellular inflammatory responses. It shares us of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28, IL-29. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems.IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1.

Alternative Names: TIFA; MGC20791; T2BP; T6BP; TIFAA; TRAF-interacting protein with a forkhead-associated domain; TRAF2 binding protein; TRAF6 binding protein

Search name: TIFA; MGC20791; T2BP; T6BP; TIFAA; TRAF-interacting protein with a forkhead-associated domain; TRAF2 binding protein; TRAF6 binding protein

Tag: TIFA