Mouse Trehalase (TREH) ELISA Kit | KTE70059

Mouse Trehalase (TREH) ELISA Kit | KTE70059 | Gentaur UK, US & Europe Distribution

Application: This Mouse Trehalase (TREH) ELISA Kit employs a two-site sandwich ELISA to quantitate TREH in samples. An antibody specific for TREH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTREH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TREH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TREH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Trehalase microplate
• Mouse Trehalase standard
• Mouse Trehalase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Trehalase (TREH) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TREH. No significant cross-reactivity or interference between Mouse TREH and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Trehalose (1-alpha-D-glucopyranosyl alpha-D-glucopyranoside) is widely distributed in nature, being the storage disaccharide of fungi and the blood sugar of insects. Trehalase (EC 3.2.1.28) is an intrinsic glycoprotein of the small intestine and renal brush border membranes that hydrolyzes trehalose to 2 glucose molecules. The deduced 583-amino acid protein has a calculated molecular mass of 66.6 kD. It has an N-terminal signal peptide, 5 potential N-glycosylation sites, and a C-terminal hydrophobic region for glycosylphosphatidylinositol (GPI) attachment. Northern blot analysis detected a 2.0-kb transcript mainly in kidney, liver, and small intestine.The trehalase signature sequence from amino acids 162 to 175 of mouse Treh shares 100% identity with the corresponding region in human TREH.

Alternative Names: TREH; MGC129621; TRE; TREA; alpha;alpha-trehalase; alpha;alpha-trehalose glucohydrolase; trehalase

Search name: TREH; MGC129621; TRE; TREA; alpha;alpha-trehalase; alpha;alpha-trehalose glucohydrolase; trehalase

Tag: TREH