Mouse TRH (Thyrotropin Releasing Hormone) ELISA Kit | ELK2642

Mouse TRH (Thyrotropin Releasing Hormone) ELISA Kit | ELK2642 | Gentaur UK, US & Europe Distribution

Alternative Names: TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor

Species: Mouse

Assay Type: Competitive Inhibition

Sensitivity: 2.63 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology;Neuro science;Hepatology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyrotropin Releasing Hormone (TRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyrotropin Releasing Hormone (TRH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyrotropin Releasing Hormone (TRH) in the samples is then determined by comparing the OD of the samples to the standard curve.