Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) ELISA Kit | AE60531MO

Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) ELISA Kit | AE60531MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: LSM5

Alternative Name: FLJ12710; YER146W; LSM5 homolog; U6 small nuclear RNA associated|OTTHUMP00000202663|OTTHUMP00000202664|OTTHUMP00000202667|U6 snRNA-associated Sm-like protein

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.053 ng/mL

Intra-Assay: ≤3.7%

Inter-Assay: ≤6.5%

Recovery: 0, 98

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate LSM5 in samples. An antibody specific for LSM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLSM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LSM5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LSM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family . Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.Disruption of genes encoding Sm-like proteins directly associated with the U6 snRNA (Lsm2-8) generated variable phenotypes. Lsm2, Lsm3, Lsm4, and Lsm8 are essential for vegetative growth. Lsm5, Lsm6, and Lsm7 are not essential for growth; however, their disruptions lead to slow growth especially at elevated temperature. The levels of the U6 snRNA were strongly reduced in the strains harboring the Lsm5, Lsm6, and Lsm7 disruptions.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .