Mouse Urokinase plasminogen activator (uPA) ELISA Kit | KTE70733

Mouse Urokinase plasminogen activator (uPA) ELISA Kit | KTE70733 | Gentaur UK, US & Europe Distribution

Application: This Mouse Urokinase plasminogen activator (uPA) ELISA Kit employs a two-site sandwich ELISA to quantitate PLAU in samples. An antibody specific for PLAU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLAU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAU is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAU bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Urokinase plasminogen activator microplate
• Mouse Urokinase plasminogen activator standard
• Mouse Urokinase plasminogen activator detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Urokinase plasminogen activator (uPA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PLAU. No significant cross-reactivity or interference between Mouse PLAU and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Urokinase-type plasminogen activator is a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. The protein encoded by this gene converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. This gene's proprotein is cleaved at a Lys-Ile bond by plasmin to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA) . HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor.

Alternative Names: PLAU; ATF; UPA; URK; u-PA; U-plasminogen activator; plasminogen activator; urinary; urokinase-type plasminogen activator

Search name: PLAU; ATF; UPA; URK; u-PA; U-plasminogen activator; plasminogen activator; urinary; urokinase-type plasminogen activator

Tag: PLAU