Mouse Vesicular glutamate transporter 1 (SLC17A7) ELISA Kit | KTE70432

Mouse Vesicular glutamate transporter 1 (SLC17A7) ELISA Kit | KTE70432 | Gentaur UK, US & Europe Distribution

Application: This Mouse Vesicular glutamate transporter 1 (SLC17A7) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC17A7 in samples. An antibody specific for SLC17A7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC17A7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC17A7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC17A7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Vesicular glutamate transporter 1 microplate
• Mouse Vesicular glutamate transporter 1 standard
• Mouse Vesicular glutamate transporter 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Vesicular glutamate transporter 1 (SLC17A7) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SLC17A7. No significant cross-reactivity or interference between Mouse SLC17A7 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: SLC17A7 is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

Alternative Names: SLC17A7; BNPI; VGLUT1; brain-specific Na-dependent inorganic phosphate cotransporter; solute carrier family 17; member 7; vesicular glutamate transporter 1

Search name: SLC17A7; BNPI; VGLUT1; brain-specific Na-dependent inorganic phosphate cotransporter; solute carrier family 17; member 7; vesicular glutamate transporter 1

Tag: SLC17A7