(Mouse) Zcchc11 Antibody (C-term) [APR32940G]

(Mouse) Zcchc11 Antibody (C-term) [APR32940G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This (Mouse) Zcchc11 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1568-1600 amino acids from the C-terminal region of (Mouse) Zcchc11.

Cellular Localisation: Nucleus {ECO:0000250|UniProtKB:Q5TAX3}. Cytoplasm. Cytoplasm, Cytoplasmic ribonucleoprotein granule {ECO:0000250|UniProtKB:Q5TAX3}. Note=Mainly cytoplasmic (PubMed:19703396) . Translocates into the cytoplasm following treatment of the cell with LPS. Co-enriched in cytoplasmic foci with MOV10 {ECO:0000250|UniProtKB:Q5TAX3, ECO:0000269|PubMed:19703396}

Molecular Weight: 184650

Clone: Polyclonal

Gene Name: Tut4 {ECO:0000312|MGI:MGI:2445126}

Gene ID: 230594

Function: Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly (A) tails, hence facilitating global mRNA decay (PubMed:28792939) . Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (PubMed:28792939) . Involved in microRNA (miRNA) -induced gene silencing through uridylation of deadenylated miRNA targets. Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (By similarity) . Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency (By similarity) . Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (PubMed:19703396) . In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:28671666) . Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (By similarity) . May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP (By similarity) . Does not play a role in replication- dependent histone mRNA degradation (By similarity) . Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:28671666, PubMed:28792939, PubMed:22898984) . TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (By similarity) .

Summary: Tissue Location: Ubiquitously expressed.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Terminal uridylyltransferase 4, TUTase 4, Zinc finger CCHC domain-containing protein 11, Zcchc11, Kiaa0191, Tut4