Mouse Zinc transporter 4 (SLC30A4) ELISA Kit | KTE70426

Mouse Zinc transporter 4 (SLC30A4) ELISA Kit | KTE70426 | Gentaur UK, US & Europe Distribution

Application: This Mouse Zinc transporter 4 (SLC30A4) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC30A4 in samples. An antibody specific for SLC30A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC30A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Zinc transporter 4 microplate
• Mouse Zinc transporter 4 standard
• Mouse Zinc transporter 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Zinc transporter 4 (SLC30A4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SLC30A4. No significant cross-reactivity or interference between Mouse SLC30A4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Zinc is the second most abundant trace metal in the human body. It is an essential element, serving both a structural role, as in the formation of zinc fingers in DNA-binding proteins, and a catalytic role in metalloenzymes, such as pancreatic carboxypeptidases, alkaline phosphatases, various dehydrogenases, and superoxide dismutases. SLC30A4, or ZNT4, belongs to the ZNT family of zinc transporters. ZNTs are involved in transporting zinc out of the cytoplasm and have similar structures, consisting of 6 transmembrane domains and a histidine-rich cytoplasmic loop . The gene encodes a 430-amino acid protein that is homologous to Znt2 (SLC30A2) and Znt3 (SLC30A3) . Znt4 was abundantly expressed in mammary epithelia and brain.

Alternative Names: SLC30A4; ZNT4;

Search name: SLC30A4; ZNT4;

Tag: SLC30A4