Mouse Zinc transporter 5 (SLC30A5) ELISA Kit | AE18743MO

Mouse Zinc transporter 5 (SLC30A5) ELISA Kit | AE18743MO | Gentaur UK, US & Europe Distribution

Species Reactivity: Mouse (Mus musculus)

Abbreviation: SLC30A5

Alternative Name: FLJ12496; FLJ12756; MGC5499; ZNT5; ZNTL1; ZTL1; ZnT-5; zinc transporter 5|zinc transporter ZTL1

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.108 ng/mL

Intra-Assay: ≤4.9%

Inter-Assay: ≤9.8%

Recovery: 0, 92

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SLC30A5 in samples. An antibody specific for SLC30A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC30A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The deduced 523-amino acid protein contains 12 predicted membrane-spanning domains with extracellular N and C termini. Between transmembrane domains 9 and 10, ZTL1 has a zinc-binding histidine-rich intracellular loop. ZTL1 shares 34% identity with ZNT1 (SLC30A1) over a 122-amino acid stretch, and it shares significant homology with several rodent zinc transporters. Northern blot analysis detected 2.0- and 5.0-kb transcripts in CaCo2 cell line RNA. RT-PCR of mouse tissues detected ubiquitous expression, with highest level in kidney and lowest level in liver.The deduced 765-amino acid protein has 15 predicted membrane-spanning domains and a calculated molecular mass of about 84 kD. Western blot analysis of the full-length protein expressed in HeLa cells indicated an apparent molecular mass of about 55 kD.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .