Mouse Zinc transporter 5 (SLC30A5) ELISA Kit | KTE70425

Mouse Zinc transporter 5 (SLC30A5) ELISA Kit | KTE70425 | Gentaur UK, US & Europe Distribution

Application: This Mouse Zinc transporter 5 (SLC30A5) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC30A5 in samples. An antibody specific for SLC30A5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC30A5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Zinc transporter 5 microplate
• Mouse Zinc transporter 5 standard
• Mouse Zinc transporter 5 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Zinc transporter 5 (SLC30A5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SLC30A5. No significant cross-reactivity or interference between Mouse SLC30A5 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The deduced 523-amino acid protein contains 12 predicted membrane-spanning domains with extracellular N and C termini. Between transmembrane domains 9 and 10, ZTL1 has a zinc-binding histidine-rich intracellular loop. ZTL1 shares 34% identity with ZNT1 (SLC30A1) over a 122-amino acid stretch, and it shares significant homology with several rodent zinc transporters. Northern blot analysis detected 2.0- and 5.0-kb transcripts in CaCo2 cell line RNA. RT-PCR of mouse tissues detected ubiquitous expression, with highest level in kidney and lowest level in liver.The deduced 765-amino acid protein has 15 predicted membrane-spanning domains and a calculated molecular mass of about 84 kD. Western blot analysis of the full-length protein expressed in HeLa cells indicated an apparent molecular mass of about 55 kD.

Alternative Names: SLC30A5; FLJ12496; FLJ12756; MGC5499; ZNT5; ZNTL1; ZTL1; ZnT-5; zinc transporter 5; zinc transporter ZTL1

Search name: SLC30A5; FLJ12496; FLJ12756; MGC5499; ZNT5; ZNTL1; ZTL1; ZnT-5; zinc transporter 5; zinc transporter ZTL1

Tag: SLC30A5