MPYS Antibody | 5269

MPYS Antibody | 5269 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat (82%) , Bovine: (82%) , Pig: (82%) , Chicken: (71%)

Immunogen: Anti-MPYS antibody (5269) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human MPYS.
The immunogen is located within amino acids 260-310 of MPYS.

Research Area: Innate Immunity

Tested Application: E, WB, ICC, IF

Application: WB: 1-2 μg/mL; ICC: 5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1204 - K562 Cell Lysate

Positive Control 2: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3: Cat. No. 1288 - A20 Cell Lysate

Positive Control 4: Cat. No. 17-208 - A-20 Cell Slide

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 42kD
Observed: 42 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar MPYS expression profile in human cell lines detected by two independent anti-MPYS antibodies that recognize different epitopes, 5269 against near C-terminus domain and 64-236 against C-terminus domain.  MPYS proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Isoform: Human MPYS has one isoform (379aa, 42kD) . Mouse MPYS has 3 isoforms, including isoform 1 (379aa, 42kD) , isoform 2 (427aa, 48kD) and isoform 3 (337aa, 38kD) . Rat MPYS also has one isoform (379aa, 43kD) . 5269 can detect human, mouse and rat.

Purification: MPYS Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: MPYS Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: MPYS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: MPYS Antibody: ERIS, MITA, MPYS, NET23, STING, ERIS, Stimulator of interferon genes protein, Endoplasmic reticulum interferon stimulator, hSTING

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: MPYS Antibody: MPYS is a recently identified plasma membrane tetraspanner that is associated with major histocompatibility complex class II (MHC-II) and mediates its transduction of apoptotic signals. It has also been found to be associated with VISA, a mitochondrial protein that acts as an adaptor in virus-triggered signaling. MPYS also interacts with IRF3 and recruits the kinase TBK1 to the VISA-associated complex, acting as a critical mediator of virus-triggered IRF3 activation and interferon (IFN) expression. It is thought that the binding of nucleic acid to the innate immune protein RIG-I causes complex formation between RIG-I, VISA, and MPYS. This complex then recruits TBK1 to phosphorylate IRF3 which then directly activates IFN transcription. At least three isoforms of MPYS are known to exist.