MRCK beta, Active | 7772

Myotonic Dystrophy Kinase-Related cdc42-binding kinase (MRCKb) belongs to the DMPK subfamily (1). The myotonic dystrophy kinase-related kinases and myotonic dystrophy kinase-related Cdc42 binding kinase (MRCK) are effectors of RhoA and Cdc42, respectively, where they are involved in actin cytoskeletal reorganization and neurite outgrowth (2). Effects of the repeat expansion on the DMPK gene may be responsible for muscle and heart features of Myotonic Dystrophy.

7772 | MRCK beta Active DataSheet

Biomolecule/Target: N/A

Synonyms: MRCK, CDC42 binding protein kinase beta (DMPK-like)

Alternates names: MRCK, CDC42 binding protein kinase beta (DMPK-like), DMPK-like beta, Myotonic dystrophy kinase-related CDC42-binding kinase beta

Taglines: Involved in the regulation of cytoskeleton reorganization, cell migration, wound healing and invasion

NCBI Gene ID #: 6351, 9559

NCBI Gene Symbol: CCL4

Gene Source: N/A

Accession #: P13236

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 99%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity of MRCKB was determined to be 120 nmol /min/mg as per activity assay protocol.

Biological activity: Determined by its ability to chemoattract human blood monocytes using a concentration of 5.0-20 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 73.0 kDa

Concentration: 0.1mg/ml

Appearance: Liquid

Physical form description: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A