MRPS12 Antibody | 13-731

MRPS12 Antibody | 13-731 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-138 of human MRPS12 (NP_203527.1) .

Research Area: Other

Tested Application: WB, IHC

Application: WB: 1:1000 - 1:2000
IHC: 1:50 - 1:200

Specificiy: N/A

Positive Control 1: LO2

Positive Control 2: U-251MG

Positive Control 3: HT-29

Positive Control 4: 293T

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Observed: 14kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: MRPS12, MPR-S12, MT-RPS12, RPMS12, RPS12, RPSM12

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4) . Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein.