MS | Malate synthase, (glyoxysomal) | AS19 4295

MS | Malate synthase, (glyoxysomal) | AS19 4295 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide derived from Cucurbita maxima UniProt: P24571

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: Collection of antibodies for carbohydrate metabolism

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 65 kDa

Confirmed Reactivity: Nicotiana tabacum

Predicted Reactivity: Arabidopsis thaliana, Cajanus cajan, Cinnamomum micranthum f. kanehirae, Cucumis sativus, Cucurbita maxima, Fagus sylvatica, Glycine max, Jatropha curcas, Morus notabilis, Mucuna pruriens, Parasponia andersonii, Populus alba x tremula, Theobroma cacao, Trema orientale Species of your interest not listed? Contact us

Not reactive in: Sorghum bicolor

Additional Information: Experimental contitions: 5 µg of total protein extracted freshly from 3-4 weeks old plant leaves with a blender at 4 °C in 300 mM Sorbitol, 50 mM HEPES, 5mM MgCl2. Separated on 10 % SDS-PAGE and blotted 1h to PVDF, semi-dry. Blot was blocked with 6 % milk for 1h 4°C with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 ON at 4°C with agitation. According to South et. al (2019) .

Background: Malate synthase is involved in the synthesis of (S) -malate from isocitrate, where it catalyses the reaction: acetyl-CoA + glyoxylate + H (2) O = (S) -malate + CoA + H (+) . This is part of the glyoxylate cycle, which is itself part of carbohydrate metabolism.

Reconstitution: For reconstitution add 50 µl, of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: TAIR

Category: Nicotiana tabacum

Research Area: Plant Biology