MSH2 monoclonal Antibody | MB9366

MSH2 monoclonal Antibody | MB9366 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Rat

Application: WB IHC

Application Range: WB:1:2,000-1:5,000 IHC:1:100-1:500

Background: Component of the post-replicative DNA mismatch repair system (MMR) . Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containg DNA strand. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP>ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: MSH2 monoclonal Antibody detects endogenous levels of MSH2 protein.

Molecular Weight: 104 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: hMSH2, MSH2, BAT26, COCA 1, COCA1, DNA mismatch repair protein Msh2, FCC 1, FCC1, hMSH2, HNPCC 1, HNPCC, HNPCC1, LCFS2, MSH 2, Msh2, MSH2, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2,

Immunogen: Synthetic peptide within N-terminal residues of Human MSH2.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: ProG purified.

Pathway: