MSLN (Human) ELISA Kit - E5003

E5003 | MSLN (Human) ELISA Kit DataSheet

Mesothelin (MSLN) is a glycosylphosphatidylinositol-linked membrane protein that is expressed on mesothelial cells lining the pleura, pericardium, and peritoneum. The gene encodes a 71 kDa precursor protein that is processed to yield a 31 kDa shed protein called megakaryocyte potentiating factor (MPF) and a 40 kDa protein called Mesothelin which is attached to the cell membrane via a glycosylphosphatidylinositol anchor. Although the biological functions are unclear, several studies have demonstrated that overexpression of MSLN is involved in cell adhesion and tumor invasion. Overexpression of MSLN has been observed in several cancers such as mesothelioma, ovarian cancer, pancreatic and lung adenocarcinoma. Due to its high expression in tumors, MSLN could serve as a potential target for anti-cancer therapy. Current strategies involve developing CAR-T therapy and monoclonal antibodies that target MSLN. ’s MSLN (Human) ELISA kit quantitatively measures MSLN in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of MSLN in the sample or standard.

Alternate Name : MPF, SMRP, MSLN, CAK1

Tag Line : A Sandwich ELISA kit for quantitative measurement of Mesothelin (MSLN) in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma Serum Tissue lysate Other biological fluids

Species Reactivity : Human

Applications : Sandwich ELISA kit for quantitative measurement of MSLN in serum, plasma, cell and tissue lysates, and other biological fluids

Features and Benefits : Recovery range: 85 - 105% for normal human serum and plasma samples Detection range: 0.156 – 10 ng/ml Assay Precision: Intra-Assay CV and Inter-Assay CV < 10% This Sandwich ELISA is highly sensitive and highly specific for the detection of MSLN in human samples. There is no significant cross-reactivity or interference between MSLN and analogues Sensitivity: 0.094 ng/ml