MTMR15 Antibody (N-term) [APR32144G]

MTMR15 Antibody (N-term) [APR32144G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This MTMRF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the N-terminal region of human MTMRF.

Cellular Localisation: Nucleus Note=Localizes at sites of DNA damage following recruitment by monoubiquitinated FANCD2 (PubMed:20603015, PubMed:20603016) . Localizes to stalled replication forks via its UBZ4-type zinc finger (PubMed:20935496) .

Molecular Weight: 114225

Clone: Polyclonal

Gene Name: FAN1 {ECO:0000303|PubMed:20603015}

Gene ID: 22909

Function: Nuclease required for the repair of DNA interstrand cross- links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:20671156, PubMed:24981866, PubMed:25430771) . Not involved in DNA double-strand breaks resection (PubMed:20603015, PubMed:20603016) . Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL (PubMed:25430771) . Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (PubMed:25430771) . Also has endonuclease activity toward 5'-flaps (PubMed:20603015, PubMed:20603016, PubMed:24981866) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, FC

Dilution: WB--1:1000 FC--1:10~50

Synonyms: Fanconi-associated nuclease 1, 3121-, FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15, FAN1, KIAA1018, MTMR15