MURF2 Antibody | 45-919

MURF2 Antibody | 45-919 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig

Immunogen: The immunogen for this antibody is: C-HIFSFSWLNSLNE

Research Area: Cancer

Tested Application: E, WB, IHC, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 58kDa band observed in lysates of cell line HeLa (calculated MW of 60.5kDa according to NP_908973.1) . Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Pig, Rat and Mouse Heart, and Pig and Mouse Skeletal Muscle for this particular batch.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.) .

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: TRIM55, RNF29, MURF-2, ring finger protein 29, for muscle specific ring finger 2, tripartite motif-containing 55, muscle specific ring finger 2

User Note: N/A

BACKGROUND: N/A