Mutant Alanine Racemase Y354N, Active, Recombinant | P1116

Mutant Alanine Racemase (Y354N) (mAR-Y354N), a pyridoxal 5-phosphate (PLP) dependent enzyme catalyzes the interconversion of the L-Serine to D-Serine. In WT Alanine Racemase Tyr354 plays a crucial role in defining the strict specificity of AR for alanine, in converting L-Alanine to D-Alanine, which is an important component of the peptidoglycan layer of bacterial cell wall. By mutating the active site Tyr 354 to Asn, the specificity of the enzyme changes and it becomes a racemase with dual specificity for L- Alanine and L-Serine.

P1116 | Mutant Alanine Racemase Y354N Active Recombinant DataSheet

Biomolecule/Target: alr

Synonyms: Alanine Racemase Y354N, alr, dal

Alternates names: Protein A/G

Taglines: Catalyzes the interconversion of L-alanine and D-alanine. Also weakly active on serine.

NCBI Gene ID #: N/A

NCBI Gene Symbol: N/A

Gene Source: N/A

Accession #: N/A

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One Unit enzyme converts 1 µmole of L-serine into D-serine per minute at 37 ºC and pH 7.4 in the presence of pyridoxal phosphate

Molecular Weight: 59.7 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized form proprietary buffer

Reconstitution Instructions: Reconstitution in HO to a concentration of 5 mg/ml gives a clear solution.

Amino acid sequence: N/A