Myeloperoxidase polyclonal Antibody | BS90902

Myeloperoxidase polyclonal Antibody | BS90902 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB ICC/IF IHC

Application Range: WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200

Background: The heme protein myeloperoxidase (MPO) is a major component of azurophilic granules of neutrophils and polymorphonuclear leukocytes. Optimal oxygen-dependent microbiocidal activity depends on MPO as the critical enzyme for the generation of hypochlorous acid and other toxic oxygen products. The MPO precursor is synthesized during the promyelocytic stage of myeloid differentiation and is subsequently processed and transported intracellularly to the lysosomes. The precursor undergoes cotranslational N-linked glycosylation to produce a glycoprotein. Glucosidases in the endoplasmic reticulum (ER) or early cis Golgi convert the pro-MPO to a form which is sorted into a prelysosomal compartment, which undergoes final proteolytic maturation to native MPO, a pair of heavy-light protomers. In normal neutrophils, MPO is expressed as a dimer. Calreticulin, a calcium-binding protein residing in the ER, interacts specifically with fully glycosylated apopro-MPO. iMPO mRNA is abundant in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60 cells. MPO is expressed at high levels in circulating neutrophils and monocytes but is not detectable in microglia, brain-specific macrophages or normal brain tissue.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Myeloperoxidase polyclonal Antibody detects endogenous levels of Myeloperoxidase protein.

Molecular Weight: 84 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: MPO, 84 kDa myeloperoxidase, 89 kDa myeloperoxidase, EC 1.11.1.7, EC1.11.2.2, fj80f04, MPO, mpx, myeloid-specific peroxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain, PERM, wu:fj80f04,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: ProA affinity purified

Pathway: