MYLK3 Antibody| F40165-0.08ML/F40165-0.4ML

MYLK3 Antibody| F40165-0.08ML/F40165-0.4ML | Gentaur UK, US & Europe Distribution

Background: MLCK/MYLK3, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

Format: Purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human, Mouse

Predicted Spacies Reactivity: N/A

Application: IHC, WB, ELISA

Application Details: Western blot: 1:1000, IHC (Paraffin): 1:50-1:100

Application Note: Titration of the MYLK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This MYLK3 antibody is available for research use only.

Purity: Purified

Immunogen: A portion of amino acids 40-69 from the human protein was used as the immunogen for this MYLK3 antibody.

Storage: Aliquot the MYLK3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A