MyoGEF Antibody (N-term) [APR32354G]

MyoGEF Antibody (N-term) [APR32354G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This MyoGEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-87 amino acids from the N-terminal region of human MyoGEF.

Cellular Localisation: Cell projection, microvillus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cleavage furrow. Note=During mitosis, localizes to the spindle pole, central spindle and cleavage furrow (PubMed:16721066) . In epithelial cells, recruited to the apical membrane by EZR where it participates in macropinocytosis (PubMed:17881735)

Molecular Weight: 88960

Clone: Polyclonal

Gene Name: PLEKHG6

Gene ID: 55200

Function: Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. Also activates RHOG. Does not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis.

Summary: Tissue Location: Highest expression in the placenta. Low levels in small intestine, lung, liver, kidney, thymus and heart

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, FC

Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50

Synonyms: Pleckstrin homology domain-containing family G member 6, PH domain-containing family G member 6, Myosin-interacting guanine nucleotide exchange factor, MyoGEF, PLEKHG6