NAE1 Antibody (Center) [APR03771G]

NAE1 Antibody (Center) [APR03771G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This NAE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 172-200 amino acids from the Central region of human NAE1.

Cellular Localisation: Cell membrane. Note=Colocalizes with APP in lipid rafts

Molecular Weight: 60246

Clone: Polyclonal

Gene Name: NAE1

Gene ID: 8883

Function: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

Summary: Tissue Location: Ubiquitous in fetal tissues. Expressed throughout the adult brain.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1