NALP1 Antibody [Nalpy1-4] | XA-1024

NALP1 Antibody [Nalpy1-4] | XA-1024 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: NALP1 (monoclonal Nalpy1-4) antibody was raised against recombinant human NALP1 (NACHT-, LRR- and PYD-) containing protein.

Research Area: Apoptosis

Tested Application: WB, IHC, ICC, IP

Application: NALP1 can be used to recognize the pyrin domain (PYD) of human NALP1 by Immunocytochemistry (1:1, 000) , Immunohistochemistry (frozen sections, paraffin sections (microwave treatment) ) , Immunoprecipitation, and Western Blot. Method: Assembly of the inflammasome was induced by shifting the temperature to 30˚C after hypotonic lysis. THP-1 cell extracts were immunoprecipitated with PAb to Asc (AL177) and run on SDS-PAGE. NALP1 was detected by MAb to NALP1 (Nalpy1-4) at 1:1'000 dilution. Anti-mouse IgG coupled horse radish peroxidase was used at 1:5, 000 dilution for ECL detection. For more information about the "time course assembly of inflammasome" method see F. Martinon; Mol. Cell. 10, 417 (2002) . Method: 293T cells transfected with full length human NALP1 were fixed with 4% paraformaldehyde and stained with 1 μg/mL MAb to NALP1 (Nalpy1-4) and then Alexa 488 (1:300 dilution) . This product is for research use only.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: NALP1 (monoclonal Nalpy1-4) antibody was purified from concentrated hybridoma tissue culture supernatant. NALP1 (monoclonal Nalpy1-4) antibody was purified to >=95% in SDS-PAGE.

Clonality: Monoclonal

Clone: Nalpy1-4

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS containing 0.02% sodium azide

Concentration: N/A

Storage Condition: NALP1 antibody can be stored at 4˚C, stable for one year.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: N/A