NAT10 Antibody (N-Term) [APR33603G]

NAT10 Antibody (N-Term) [APR33603G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This NAT10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 146-178 amino acids from the mouse region of mouse NAT10.

Cellular Localisation: Nucleus, nucleolus {ECO:0000255|HAMAP- Rule:MF_03211, ECO:0000269|PubMed:12429849, ECO:0000269|PubMed:14592445, ECO:0000269|PubMed:19303003, ECO:0000269|PubMed:24786082, ECO:0000269|PubMed:25653167, ECO:0000269|PubMed:30165671}. Midbody {ECO:0000255|HAMAP-Rule:MF_03211, ECO:0000269|PubMed:19303003}. Note=Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase.

Molecular Weight: 115730

Clone: Polyclonal

Gene Name: NAT10 {ECO:0000255|HAMAP-Rule:MF_03211}

Gene ID: 55226

Function: RNA cytidine acetyltransferase that catalyzes the formation of N (4) -acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs (PubMed:25411247, PubMed:25653167, PubMed:30449621) . Catalyzes ac4C modification of a broad range of mRNAs, enhancing mRNA stability and translation (PubMed:30449621) . mRNA ac4C modification is frequently present within wobble cytidine sites and promotes translation efficiency (PubMed:30449621) . Mediates the formation of ac4C at position 1842 in 18S rRNA (PubMed:25411247) . May also catalyze the formation of ac4C at position 1337 in 18S rRNA (By similarity) . Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis (PubMed:25411247, PubMed:25653167) . Catalyzes the formation of ac4C in serine and leucine tRNAs (By similarity) . Requires the tRNA-binding adapter protein THUMPD1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation (PubMed:25653167) . In addition to RNA acetyltransferase activity, also able to acetylate lysine residues of proteins, such as histones, microtubules, p53/TP53 and MDM2, in vitro (PubMed:14592445, PubMed:17631499, PubMed:19303003, PubMed:26882543, PubMed:27993683, PubMed:30165671) . The relevance of the protein lysine acetyltransferase activity is however unsure in vivo (PubMed:30449621) . Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization (PubMed:14592445, PubMed:18082603) . Involved in the regulation of centrosome duplication by acetylating CENATAC during mitosis, promoting SASS6 proteasome degradation (PubMed:31722219) .

Summary: N/A

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB, FC

Dilution: FC--1:25 WB--1:2000

Synonyms: N-acetyltransferase 10, 2.3.1.-, NAT10, ALP, KIAA1709