NCF1 Antibody | 45-096

NCF1 Antibody | 45-096 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse, Pig

Homology: Expected Species Reactivity based on sequence homology: Rat, Cow

Immunogen: The immunogen for this antibody is: C-SESTKRKLASAV

Research Area: Homeostasis

Tested Application: E, WB, IHC, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:20000.Western Blot:Approx 48kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50kDa in Pig Spleen lysates (calculated MW of 44.7kDa according to Human NP_000256.4, 46.0kDa according to Mouse NP_001272966.1 and 45.3kDa according to Pig NP_001106691.1) . The observed molecular weights correspond to different antibodies from other commercial sources. Recommended concentration: 0.01-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate. Preliminary testing was unsuccessful on Rat Spleen and Thymus for this particular batch.Immunohistochemistry:Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificiy: N/A

Positive Control 1: Cat. No. 1215 - U937 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD) , neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1) , NADPH oxidase organizer 2

User Note: N/A

BACKGROUND: N/A