NCOA4 (IN) Antibody | 9663

NCOA4 (IN) Antibody | 9663 | Gentaur UK, US & Europe Distribution

Product Type: Primary Antibodies

Category: Primary Antibodies, Polyclonal Antibodies, Signal Transduction Antibodies

Research Area: Signal Transduction

Type: Polyclonal

Host Species: Rabbit

Reactivity Species: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: monkey (100%), gorilla (100%), dog (100%), horse (100%)

Immunology: Anti-NCOA4 antibody (9663) was raised against a peptide corresponding to 13 amino acids near the center of human NCOA4. The immunogen is located within 420-470 amino acids of NCOA4.

Tested Application: E, IHC, WB, IF

Application Note: WB: 0.5-1 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

Positive Control 1: Cat. No. 1202 – Daudi Cell Lysate

Positive Control 2: Cat. No. 1402 – Mouse Lung Lysate

Predicted Molecular Weight: Predicted: 70-74 kD

Observed: 70-74 kD

Isoform: Human NCOA4 has 4 isoforms, including isoform alpha (614aa, 69.7kD), isoform beta (287aa, 32.6kD), isoform 3 (630aa, 71.4kD) and isoform 4 (650aa, 73.6kD) . Mouse NCOA4 has at least one isoform (625aa, 70.4kD) . Rat NCOA4 has one isoform (622aa, 70.3kD) . 9663 can detect NCOA4 in human, mouse and rat.

Purification: NCOA4 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NCOA4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage: NCOA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Names: NCOA4 Antibody: Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, Androgen receptor-associated protein of 70 kDa, Ret-activating protein ELE1, ARA70, ELE1, RFG

Protein Accession: Q13772

User Note: Optimal dilutions for each application to be determined by the researcher.

Background: NCOA4 Antibody: Study showed that NCOA4-mediated ferritin complex degradation supplies iron to mitochondria. Loss of COPZ1 induces NCOA4 mediated autophagy and ferroptosis in glioblastoma cell lines. NCOA4-mediated ferritinophagy promoted inflammatory responses in periodontitis. Low expression of ferritinophagy-related NCOA4 gene in relation to unfavorable outcome and defective immune cells infiltration in clear cell renal carcinoma.

Loading Controls: N/A

Source: N/A

Purity: N/A

CAS: N/A

Shipping Condition: N/A