NdbA | Thylakoid Localized Type 2 NAD (P) H Dehydrogenase | AS19 4297

NdbA | Thylakoid Localized Type 2 NAD (P) H Dehydrogenase | AS19 4297 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide derived from NdbA protein sequence of Synechocystis sp. PCC 6803, UniProt:P73739 Chosen peptide is not conserved in NdbB and NdbC.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: collection of antibodies to cyanobacterial proteins

Recommended Dilutions: 1 : 2000 (WB)

Molecular weight: 49 | 46 kDa

Confirmed Reactivity: Synechocystis sp. PCC 68

Predicted Reactivity: Bacillus subtilis Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Protein extraction: harvested cells were suspended in a buffer containing 50 mM Hepes-NaOH, pH 7, 5, 30 mM CaCl2, 800 mM sorbitol, 1 mM ε-amino-n-caproic acid, and the cells were broken by vortexing 6×1 min at 4°C in the presence of glass beads, Protein samples were solubilized in Laemmli buffer containing 6 M urea and separated on a gel in presence of 6M urea

Background: NdbA (Thylakoid Localized Type 2 NAD (P) H Dehydrogenase) is localized to the thylakoid membrane and expressed at a very low level in the widl type under photoautotrophic growth. NdbA is induced substantially under light-activated heterotophic growth conditions.

Reconstitution: For reconstitution add 50 µl, of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Electron transfer

Research Area: Plant Biology, Photosynthesis