NEDD8-AMC | 4843

Prepared by the C-terminal derivatisation of NEDD8 with 7-amino-4-methylcoumarin. NEDD8-AMC acts as a useful and sensitive fluorogenic substrate for deNEDDylating enzymes, including NEDP1 and UCH-L3. It is a useful reagent for the study of deNEDDylating activity where detection sensitivity or continuous monitoring of activity is essential.

4843 | NEDD8-AMC DataSheet

Biomolecule/Target: N/A

Synonyms: NEDD8-Aminomethylcoumarin

Alternates names: NEDD8-Aminomethylcoumarin

Taglines: A protein implicated in cell cycle progression and cytoskeletal regulation

NCBI Gene ID #: 6351, 9560

NCBI Gene Symbol: CCL4

Gene Source: Human

Accession #: P13236

Recombinant: Yes

Source: N/A

Purity by SDS-PAGEs: 98%

Assay: N/A

Purity: 95%

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract human blood monocytes using a concentration of 0.1-1 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 8.735 kDa

Concentration: 1 mg/ml (DMSO)

Appearance: Liquid

Physical form description: A solution in DMSO ( 1 mg/ml)

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Amino acid sequence: N/A