NEK2, Active | 7719

Nek 2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the Nek2 protein is almost undetectable during G1 but accumulated progressively throughout S, reaching maximal levels in late G2 (1). These observations demonstrate that Nek2 resembles Aspergillus NIMA, not only in its catalytic domain, but also in its cell cycle-dependent expression. Recombinant Nek2 is active as a serine/threonine-specific protein kinase and may undergo autophosphorylation. Both human Nek2 and fungal NIMA phosphorylate a similar, albeit not identical, set of proteins and synthetic peptides, and -casein is a suitable substrate for assaying Nek2 in vitro (2). Nek2 is shown to be expressed most abundantly in the testis of the adult tissues examined (3). Its expression in the testis is restricted to the germ cells, with highest levels detected in spermatocytes at pachytene and diplotene stages. Immunohistochemical analysis revealed that Nek2 localized to nuclei, exhibiting a non-uniform distribution within the nucleus.

7719 | NEK2 Active DataSheet

Biomolecule/Target: N/A

Synonyms: NEK, Serine/threonine-protein kinase Nek6

Alternates names: NEK, Serine/threonine-protein kinase Nek6, Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512

Taglines: Kinase involved in cell cycle, checkpoint control and cancer

NCBI Gene ID #: 20310

NCBI Gene Symbol: MIP2

Gene Source: N/A

Accession #: P10889

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 183 nmol phosphate incorporated into MBP per minute per mg protein at 300C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: Determined by measuring the dose dependent mobilization of intraCellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with >5 ng/ml of recombinant mouse MIP-2.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 76.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. The solution can be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A