NEK7, Active | 7717

Nek7 is a member of the NIMA (never in mitosis, gene A) family of serine/threonine kinases. In contrast to the other documented NIMA-related kinases, Nek7 harbor its catalytic domain in the C-terminus of the protein. Immunofluorescence studies suggest that Nek7 is cytoplasmic and located on chromosome 1 (1). During early embryogenesis Nek7 is expressed in the site of decidual reaction while later in embryogenesis, it is almost exclusively restricted to the nervous system in the dorsal thalamus (2). The major protein kinase that is active on the p70 S6 kinase hydrophobic regulatory site (FXXFS/TF/Y) Thr412 was purified from rat liver and identified as Nek7 (3). Nek7 kinase activity is rapidly and efficiently increased by serum deprivation, and may be regulated in a cell cycle-dependent manner.

7717 | NEK7 Active DataSheet

Biomolecule/Target: N/A

Synonyms: NEK, Serine/threonine-protein kinase Nek7

Alternates names: NEK, Serine/threonine-protein kinase Nek6, Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512

Taglines: Kinase involved in cell cycle, checkpoint control and cancer

NCBI Gene ID #: 6362

NCBI Gene Symbol: MIP4

Gene Source: N/A

Accession #: P55774

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 221 nmol phosphate incorporated into -casein per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The biological activity of human MIP-4 was determined by a chemotaxis assay using human T lymphocytes. Significant chemotaxis was achieved at 100 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 63.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 1 mg/ml. The solution can be diluted into other buffered solutions

Amino acid sequence: N/A