Nephrocystin-1 polyclonal Antibody | BS8539

Nephrocystin-1 polyclonal Antibody | BS8539 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IF

Application Range: WB 1:500 - 1:2000 IF 1:50 - 1:100

Background: Juvenile nephronophthisis type 1 is caused by mutations of NPHP1, the gene encoding for nephrocystin. Nephrocystin interacts with p130Cas (BCAR1), proline-rich tyrosine kinase-2 (PTK2B or Pyk2), and tensin in embryonic kidney and testis, indicating that these proteins participate in a common signaling pathway. Nephrocystin and p130Cas interact in mammalian cells and both proteins prominently localize at or near sites of cell-cell contact in polarized Madin-Darby canine kidney epithelial cells. Expression of nephrocystin results in phosphorylation of Pyk2 on Tyrosine 402 as well as activation of downstream mitogen-activated protein kinases, such as ERK1 and ERK2. Nephrocystin contains a src-homology 3 (SH3) domain, which is highly conserved throughout evolution.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Nephrocystin-1 polyclonal Antibody detects endogenous levels of Nephrocystin-1 protein.

Molecular Weight: ~ 83kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Nephrocystin1; Nephrocystin 1; Juvenile nephronophthisis 1 protein; NPHP1; NPH1

Immunogen: Recombinant full length Human Nephrocystin-1.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: