NETO1 Antibody | 6469

NETO1 Antibody | 6469 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: NETO1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NETO1.
The immunogen is located within amino acids 350 - 400 of NETO1.

Research Area: Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: NETO1 antibody can be used for detection of NETO1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: NETO1 antibody is predicted to not cross-react with other NETO protein family members.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 60 kDa
Observed: 60 kDa

Validation: N/A

Isoform: N/A

Purification: NETO1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NETO1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NETO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NETO1 Antibody: BCTL1, BTCL1, Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NETO1 Antibody: Neuropilin and tolloid-like protein 1 (NETO1) is involved in the development and/or maintenance of neuronal circuitry. It is a type I transmembrane protein that is expressed in the brain and retina. NETO1 contains one LDL-receptor class A domain and two CUB domains and is either membrane-bound or secreted. It has three alternatively spliced isoforms, the first two of which are retina-specific and the third of which is found in both retina and brain tissue. Furthermore, as an accessory subunit of the neuronal N-methyl-D-aspartate receptor (NMDAR) , it regulates long-term NMDA receptor-dependent synaptic plasticity and cognition, at least in the context of spatial learning and memory.