NEUCRIN Antibody | 6493

NEUCRIN Antibody | 6493 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Chicken: (100%) , Bovine: (100%)

Immunogen: Neucrin antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human Neucrin.
The immunogen is located within the last 50 amino acids of NEUCRIN.

Research Area: Neuroscience

Tested Application: E, WB, IHC-P, IF

Application: Neucrin antibody can be used for detection of Neucrin by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of Neucrin are known to exist; this antibody will detect the two shorter isoforms. Neucrin antibody is predicted to not cross-react with RILP.

Positive Control 1: Cat. No. 1473 - Rat Cerebellum Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: NEUCRIN Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NEUCRIN Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NEUCRIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NEUCRIN Antibody: UNQ3119, neucrin, AGPA3119, C1orf187, PSEC0258, UNQ3119/PRO10268, Draxin, Dorsal inhibitory axon guidance protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NEUCRIN Antibody: Neucrin, also known as Draxin, is a repulsive guidance protein for the spinal cord and forebrain commissures. It is thought to act as an antagonist of canonical Wnt signaling by inhibiting the stabilization of cytosolic beta-catenin. Ectopically expressed neucrin inhibited growth or caused misrouting of chick spinal cord commissural axons in vivo while Neucrin-null mice showed defasciculation of spinal cord commissural axons and an absence of all forebrain commisures. Other experiments in mice have shown that olfactory bulb axonal outgrowth is inhibited by Neucrin, suggesting that Neucrin functions as an inhibitory guidance cue for olfactory bulb axons.