Description
Neurochondrin Antibody | 45-952 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: C-HYLLQVGSEKQKEP
Research Area: Cancer
Tested Application: E, WB
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and U251 (calculated MW of 77.2kDa according to NP_001014841.1) . An additional band of unknown identity was also observed in U251 lysates at 55kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat and Mouse Kidney and Brain and NIH3T3 cell line for this particular batch.
Specificiy: This antibody is expected to recognize both reported isoforms (NP_055099.1; NP_001014841.1) . Reported variants represent identical protein (NP_055099.1; NP_001014839.1) .
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: NCDN, neurochondrin, KIAA0607
User Note: N/A
BACKGROUND: N/A