Neurofilament Heavy Antibody [RT97] | 33-180

Neurofilament Heavy Antibody [RT97] | 33-180 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat, Chicken, Pig

Homology: N/A

Immunogen: The triton-X 100 insoluble protein fraction of rat brain was used as the immunogen for this Neurofilament Heavy antibody.

Research Area: Neuroscience, Signal Transduction, Stem Cell

Tested Application: Flow, IHC

Application: Flow Cytometry: 0.5-1 ug/million cells
IHC (FFPE) : 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required) , drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: RT97

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND:

This antibody reacts with a 200kDa protein, identified as heavy subunit of neurofilaments, or NF-H. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L) , 160kDa (NF-M) and 200kDa (NF-H) , plus alpha internexin or peripherin. Each neurofilament subunit contains a globular head, an alpha helical rod domain, and variable tail domains that differ in length and amino acid content.

Neurofilament antibody stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for neurofilament antibody. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.