NF-KB p65 (Ab-536) polyclonal Antibody | BSP1257

NF-KB p65 (Ab-536) polyclonal Antibody | BSP1257 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Rat,Mouse

Application: WB

Application Range: WB: 1:2000~1:5000

Background: Transcription factors of the nuclear factor κB (NF-κB) /Rel family play a pivotal role in inflammatory and immune responses. There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52) . Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression. NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus.

Storage & Stability: Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles.

Specificity: NF-KB p65 (Ab-536)  polyclonal Antibody detects endogenous levels of NF-KB p65 protein.

Molecular Weight: ~ 65 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Immunogen: Synthetic peptide, corresponding to Human NF-KB p65.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95%

Pathway: Phosphoinosotode Signaling,Protein Kinase C Signaling,Regulation of Actin Dynamics,Warburg Effect,Regulation of Microtubule Dynamics,SAPK JNK Signaling Cascades,Inhibition of Apoptosis,G Protein-coupled Receptors Signaling to MAPK Erk,Mitochondrial Control of Apoptosis,