NHS Meningitidis RT-PCR (CE) | B25-50FRT

NHS Meningitidis RT-PCR | B25-50FRT is available for delivery

Description:

General information: Real Time PCR test for detection of N.meningitidis, H.influenzae, St.pneumonia

Target Disease Type: Neurological Infections

Specific Application: Meningitis

Storage and Shipping : stock

NHS Meningitidis RT-PCR (CE) B25-50FRT DataSheet

INTENDED USE

Kit NHS Meningitidis Real-TM is a Real-Time test for the detection and differentiation of Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae in the biological materials. DNA is extracted from specimens, amplified using RT-amplification and detected using fluorescent reporter dye probes specific for N.meningitidis, H.influenzae, S.pneumoniaeDNA and IC (Internal Control).

PRINCIPLE OF ASSAY

NHS Meningitidis Real-TM Test is based on three major processes: isolation of DNA from specimens, Real Time amplification of the DNA and NHS Meningitidis detection by the polymerase chain reaction (PCR) based on the amplification of pathogen genome specific region using specific primers and detection via fluorescent dyes. These dyes are linked with probes of oligonucleotides which bind specifically to the amplified product. The real-time PCR monitoring of fluorescence intensities allows the accumulating product detection without reopening of reaction tubes after the PCR run. NHS Meningitidis Real-TM PCR kit is a qualitative test which contain the Internal Control (IC). It must be used in the isolation procedure in order to control the process of each individual sample extraction and serves also to identify possible reaction inhibition.

STABILITY

NHS Meningitidis Real-TM test is stable up to the expiration date indicated on the kit label. The product will maintain performance through the control date printed on the label. Exposure to light, heat or humidity may affect the shelf life of some of the kit components and should be avoided. Repeated thawing and freezing of these reagents should be avoided, as this may reduce the sensitivity.