Nicotinamide N-Methyltransferase, Human Recombinant (hNNMT) | 7261

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. NNMT is an important cytosolic methyltransferase catalyzing the N-methylation of nicotinamide, pyridines and structural analogs, playing a crucial role in the biotransformation and detoxification of many xenobiotic compounds. In the N-methylation process, NNMT uses S-adenosyl methionine as the methyl donor and nicotinamide as methyl acceptor. NNMT is mostly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. It may also play an important role in regulating biological processes related to N-methyl nicotinamide such as anti-inflammatory, anti-thrombotic, vasoprotective, and gastroprotective properties. Increased NNMT activity was reported in many kinds of tumors. NNMT is a potential biomarker and therapeutic target in caner diagnosis and treatment. NNMT serum levels have significance in the premature detection and in the management of patients with colorectal cancer. The recombinant protein includes hNNMT with N-terminal His-tag.

7261 | Nicotinamide N-Methyltransferase Human Recombinant hNNMT DataSheet

Biomolecule/Target: NNMT

Synonyms: NNMT

Alternates names: Protein G

Taglines: A cytosolic methyltransferase catalyzing the N-methylation of nicotinamide

NCBI Gene ID #: N/A

NCBI Gene Symbol: N/A

Gene Source: Streptococcus sp

Accession #: CAA27638

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 26.1 kDa

Concentration: 3.42 mg/ml

Appearance: Liquid

Physical form description: 3.42 mg/ml solution in 25 mM Tris (pH8.0) buffer containing 20% glycerol

Reconstitution Instructions: Reconstitution in HO to a concentration of 5 mg/ml gives a clear solution.

Amino acid sequence: N/A