NISCH Antibody (N-Term) [ApR05012G]

NISCH Antibody (N-Term) [ApR05012G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This NISCH antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-38 amino acids from human NISCH.

Cellular Localisation: Cell membrane. Cytoplasm. Early endosome. Recycling endosome. Note=Enriched in the early/sorting and recycling endosomes. Colocalized in early/sorting endosomes with EEA1 and SNX2 and in recycling endosomes with transferrin receptor. Detected in the perinuclear region partially associated with punctate structures (By similarity) . Colocalizes with PAK1 in cytoplasm, vesicular structures in the perinuclear area and membrane ruffles (By similarity) Colocalizes with RAC1 in the cytoplasm and vesicles structures (By similarity) . Colocalized with MAPK1 and MAPK3 in RVLM neurons (By similarity) .

Molecular Weight: 166629

Clone: Polyclonal

Gene Name: NISCH

Gene ID: 11188

Function: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell- signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity) . Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity) . Acts as a modulator of Rac-regulated signal transduction pathways (By similarity) . Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (By similarity) . Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation (By similarity) . Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation (By similarity) . Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells (By similarity) . Inhibits lamellipodia formation, when overexpressed (By similarity) . Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.

Summary: Tissue Location: Isoform 1, isoform 3 and isoform 4 are expressed in brain. Isoform 1 is expressed in endocrine tissues

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: Nischarin, Imidazoline receptor 1, I-1, IR1, Imidazoline receptor antisera-selected protein, hIRAS, Imidazoline-1 receptor, I1R, Imidazoline-1 receptor candidate protein, I-1 receptor candidate protein, I1R candidate protein, NISCH, IRAS, KIAA0975