NKX2-1 Antibody | 6741

NKX2-1 Antibody | 6741 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: NKX2-1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NKX2-1.
The immunogen is located within the first 50 amino acids of NKX2-1.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: NKX2-1 antibody can be used for detection of NKX2-1 by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three.

Positive Control 1: Cat. No. 1302 - Human Lung Tissue Lysate

Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 46 kDa
Observed: 41, 45 kDa

Validation: N/A

Isoform: N/A

Purification: NKX2-1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NKX2-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NKX2-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NKX2-1 Antibody: BCH, BHC, NK-2, TEBP, TTF1, NKX2A, TITF1, TTF-1, NKX2.1, Homeobox protein NK-2 homolog A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NKX2-1 Antibody: NKX2-1 (NK2 homeobox 1) has been identified as a thyroid-specific transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype and morphogenesis. It contains one homeobox DNA-binding domain. NKX2-1 also may play a role in lung development and surfactant homeostasis. Mutations and deletions in this gene are associated with benign hereditary chorea, congenital hypothyroidism, neonatal respiratory distress, and may be associated with thyroid cancer. NKX2-1 has been suggested to be a candidate suppressor of malignant progression.