NKX2-2 Antibody | 6743

NKX2-2 Antibody | 6743 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: NKX2-2 antibody was raised against a 19 amino acid synthetic peptide near the center of human NKX2-2.
The immunogen is located within amino acids 90 - 140 of NKX2-2.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: NKX2-2 antibody can be used for detection of NKX2-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: NKX2-2 antibody is predicted to not cross-react with other NK2 homeobox family members.

Positive Control 1: Cat. No. 1465 - Rat Kidney Tissue Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: NKX2-2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NKX2-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NKX2-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NKX2-2 Antibody: NKX2-2 (NK2 homeobox 2) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-2 is crucial in the development of pancreatic islet cell differentiation and activates the ghrelin-promoter in these cells. NKX2-2 is expressed at the onset of pancreatic epithelium formation and becomes restricted to mature α-, β- and PP cells, and in NKX2-2-null mice embryos, all β-cells and most a-cells are replaced by ghrelin-positive cells. NKX2-2 is also thought to be involved in ventral neuronal patterning and floor plate development and commissural axon guidance.