NKX2-4 Antibody | 6747

NKX2-4 Antibody | 6747 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Guinea pig: (100%) , Rat: (86%) , Chicken: (86%) , Bovine: (71%)

Immunogen: NKX2-4 antibody was raised against an 18 amino acid synthetic peptide near the center of human NKX2-4.
The immunogen is located within amino acids 220 - 270 of NKX2-4.

Research Area: Stem Cell

Tested Application: E, WB, ICC, IF

Application: NKX2-4 antibody can be used for detection of NKX2-4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: NKX2-4 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-4 are known to exist; this antibody will detect both isoforms.

Positive Control 1: Cat. No. 1288 - A20 Cell Lysate

Positive Control 2: Cat. No. 17-208 - A-20 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: NKX2-4 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: NKX2-4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: NKX2-4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: NKX2-4 Antibody: NKX2D, NKX2.4, NKX2D, Homeobox protein NK-2 homolog D

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: NKX2-4 Antibody: NKX2-4 (NK2 homeobox 4) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-4 is closely related to NKX2-1 and NKX2-4 and is specifically localized to the ventral diencephalon. Recent experiments in Xenopus have suggested that the homeodomain protein XANF is necessary for the activation of NKX2-4, but does not directly regulate its expression.