NME1, human recombinant | 7823

Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas. Recombinant human NME1 was expressed in E.coli and purified by using conventional chromatography techniques.

7823 | NME1 human recombinant DataSheet

Biomolecule/Target: NME1

Synonyms: Non-metastatic cells 1, Nucleoside diphosphate kinase A, NDP kinase A, AWD, GAAD, NB, NBS, NDPK-A, NM23, NM23-H1.

Alternates names: Non-metastatic cells 1, Nucleoside diphosphate kinase A, NDP kinase A, AWD, GAAD, NB, NBS, NDPK-A, NM23, NM23-H1.

Taglines: A candidate metastasis suppressor gene

NCBI Gene ID #: 3557

NCBI Gene Symbol: IL-1RA

Gene Source: Human

Accession #: P18510

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml.

Results: Specific activity is > 180 unit/ml

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.

Molecular Weight: 17.1 kDa (152 aa, 1-152 aa)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol and 1 mM DTT

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile HO to a concentration 100 µg/ml. This solution can then be diluted into other aqueous buffers.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly.