NME2 Antibody (N-term) [APR10898G]

NME2 Antibody (N-term) [APR10898G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M, Rat

Specificity: This NME2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human NME2.

Cellular Localisation: Cytoplasm. Nucleus. Cell projection, lamellipodium. Cell projection, ruffle. Note=Isoform 3 is mainly cytoplasmic and isoform 1 and isoform 3 are excluded from the nucleolus. Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates

Molecular Weight: 17298

Clone: Polyclonal

Gene Name: NME2

Gene ID: 4831;654364

Function: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity) . Negatively regulates Rho activity by interacting with AKAP13/LBC (PubMed:15249197) . Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752, PubMed:19435876) . Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III (1) region of the MYC gene promoter. Does not bind to duplex NHE III (1) (PubMed:19435876) . Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not (PubMed:25679041) . Exhibits histidine protein kinase activity (PubMed:20946858) .

Summary: Tissue Location: Isoform 1 and isoform 3 are ubiquitously expressed.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, IF, WB

Dilution: WB--1:1000 IHC-P--1:100~500 IF--1:10~50

Synonyms: Nucleoside diphosphate kinase B, NDK B, NDP kinase B, C-myc purine-binding transcription factor PUF, Histidine protein kinase NDKB, nm23-H2, NME2, NM23B