NME3, human recombinant | 7825

NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells. Recombinant human NME3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

7825 | NME3 human recombinant DataSheet

Biomolecule/Target: NME3

Synonyms: Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3.

Alternates names: Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3.

Taglines: Important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis.

NCBI Gene ID #: 3557

NCBI Gene Symbol: IL-1RA

Gene Source: Human

Accession #: P18510

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml.

Results: Specific activity is > 20 units/ml

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.

Molecular Weight: 19.1 kDa (169 aa, 22-169 aa + His Tag)

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.1 M NaCl and 2 mM DTT

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile HO to a concentration 100 µg/ml. This solution can then be diluted into other aqueous buffers.

Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly.