NME4, human recombinant | 7826

NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Recombinant human NME4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

7826 | NME4, human recombinant DataSheet

Biomolecule/Target :NME4

Synonyms: Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D

Alternates names: Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D

Taglines: A ubiquitous enzyme that catalyze transfer of gamma-phosphates via a phosphohistidine intermediate.

NCBI Gene ID #: 4833

NCBI Gene Symbol: NME4

Gene Source: Human

Accession #: O00746

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGE: ≥90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 15 units/ml, in which one unit will convert 1.0 umole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.

Results: Specific activity is > 15 units/ml

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 umole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.

Molecular Weight: 19.6 kDa (176 aa, 33-187 aa + His Tag)

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, and 0.2 M NaCl

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans